解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Several subunits of the glutamate receptor of the AMPA subtype have been cloned recently. These subunits, named GluR1, GluR2, GluR3, and GluR4, exist as two splicing variants (flip and flop). We have determined the subset of AMPA receptor subunits expressed by single cerebellar Purkinje cells in culture. This was achi...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90164-9
更新日期:1992-08-01 00:00:00
abstract::Olfactory receptor neurons (ORNs) are replaced and differentiate in adult animals, but differentiation in dissociated cell culture has not been demonstrated. To test whether contact with the CNS regulates maturation, neonatal rat olfactory cells were grown on a culture substrate or on CNS astrocytes. Mature ORNs, immu...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90139-5
更新日期:1992-06-01 00:00:00
abstract::Several molecules involved in the development of the nervous system have specific binding sites for the glycosaminoglycan (GAG) side chains of proteoglycans. Exogenous GAGs should bind to these sites, competitively inhibit interactions with proteoglycans, and perturb development. GAGs added to the culture medium pertu...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90091-q
更新日期:1992-04-01 00:00:00
abstract::Rostrocaudal polarity of the tectum is first detectable at embryonic day 2 (E2) in the chick by the rostrocaudal gradient of en expression. When ectopic tectum is produced by heterotopic transplantation at E2 in the diencephalon, the gradient of en expression is inverted from the host polarity by environmental influen...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90099-y
更新日期:1992-04-01 00:00:00
abstract::Agrin is thought to mediate the motor neuron-induced aggregation of AChRs and AChE on the surface of muscle fibers at neuromuscular junctions. We have isolated a cDNA from a chick brain library that, based on sequence homology and expression experiments, codes for active agrin. Examination of the sequence reveals cons...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90089-v
更新日期:1992-04-01 00:00:00
abstract::The biologically active photoproduct of rhodopsin, metarhodopsin II (M II), exists in a pH-sensitive equilibrium with its precursor, metarhodopsin I (M I). Increasing acidity favors M II, with the midpoint of the pH titration curve at pH 6.4. To test the long-standing proposal that histidine protonation regulates this...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90274-h
更新日期:1992-03-01 00:00:00
abstract::We generated transgenic mice that specifically express foreign genes in myelinating Schwann cells. A 1.1 kb segment of 5' flanking sequence from the rat P0 gene was used to drive expression of the genes encoding human growth hormone (hGH) and bacterial diphtheria toxin A chain (DT-A). The P0-hGH mice expressed hGH in ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90279-m
更新日期:1992-03-01 00:00:00
abstract::von Recklinghausen's neurofibromatosis (NF1) is a common inherited human disease. The events leading to patient symptoms from inheritance of a defective NF1 gene are unknown. Since knowledge of the distribution of the normal NF1 gene product should improve understanding of the pathogenesis of the disease, we raised an...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90270-n
更新日期:1992-03-01 00:00:00
abstract::A neuron-specific cytoskeletal antigen (5E10), whose expression pattern during initial motoneuron outgrowth into the chick limb suggests that it is playing a role in axon guidance, is described. This antigen, which was shown to be a phosphorylated epitope, probably of the intermediate weight neurofilament protein (NF-...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90296-p
更新日期:1992-02-01 00:00:00
abstract::The role of cellular interactions in determining the fates of two identified motoneurons in the embryonic zebrafish was investigated by transplanting individual motoneurons from labeled donor embryos to unlabeled hosts. The results suggest that although these cells normally adopt different fates, they form an equivale...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90290-t
更新日期:1992-02-01 00:00:00
abstract::Two properties were found to distinguish neuronal from muscle nicotinic acetylcholine receptors (nAChRs). First, neuronal nAChRs have a greater Ca2+ permeability. The high Ca2+ flux through neuronal nAChRs activates a Ca(2+)-dependent Cl- conductance, and the Ca2+ to Cs+ permeability ratio (PCa/PCs) is 7 times greater...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90114-s
更新日期:1992-01-01 00:00:00
abstract::Agonist-induced suppression of current in voltage-gated Ca2+ channels was studied in rat sympathetic neurons. We have previously distinguished two intracellular signaling pathways used by muscarinic agonists to suppress neuronal Ca2+ current-one fast and membrane delimited, the other slow and acting via a diffusible s...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90111-p
更新日期:1992-01-01 00:00:00
abstract::Type IIA rat brain Na+ channel alpha subunits were expressed in CHO cells by nuclear microinjection or by transfection using a vector containing both metallothionein and bacteriophage SP6 promoters. Stable cell lines expressing Na+ channels were isolated, and whole-cell Na+ currents of 0.9-14 nA were recorded. The mea...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(92)90108-p
更新日期:1992-01-01 00:00:00
abstract::To assess the necessary parameters for the growth of axons within the adult rat CNS, we have used intracerebral grafts of primary fibroblasts genetically engineered to express nerve growth factor (NGF). Following the implantation of NGF-producing primary fibroblasts within the striatum, cholinergic axons arising from ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90346-2
更新日期:1991-12-01 00:00:00
abstract::In recent years, nerve growth factor (NGF) has gained attention as a potential therapeutic agent for Alzheimer's disease (AD). To study the expression of NGF and its homologs, brain-derived neurotrophic factor (BDNF) and neurotrophin 3 (NT-3), postmortem samples of hippocampus from AD and control donors were examined ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90273-3
更新日期:1991-11-01 00:00:00
abstract::Shaker potassium channels inactivate and recover from inactivation with multiple exponential components, suggesting the presence of multiple inactivation processes. We describe two different types of inactivation in Shaker potassium channels. N-type inactivation can occur as rapidly as a few milliseconds and has been ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90367-9
更新日期:1991-10-01 00:00:00
abstract::Olfactory receptor neurons (ORNs) do not express the typical neuronal intermediate filament proteins (IFPs), the neurofilament triplet proteins. Immunocytochemical evidence shows that ORNs coexpress vimentin and peripherin but distribute them differently. Specifically, ORNs contain vimentin in dendrites, cell bodies, ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90300-o
更新日期:1991-09-01 00:00:00
abstract::Activation of kainate receptors causes Co2+ influx into neurons, type-2 astrocytes, and O-2A progenitor cells. Agonist-activated Co2+ uptake can be performed using cultured cells or fresh tissue slices. Based on the pattern of response to kainate, glutamate, and quisqualate, three functionally different kainate-activa...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90302-g
更新日期:1991-09-01 00:00:00
abstract::We have examined the role of presynaptic residual calcium in maintaining long-term changes in synaptic efficacy observed at mossy fiber synapses between hippocampal dentate granule cells and CA3 pyramidal cells. Calcium concentrations in individual mossy fiber terminals in hippocampal slice were optically measured wit...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90297-d
更新日期:1991-09-01 00:00:00
abstract::mRNA coding for brain-derived neurotrophic factor (BDNF) has been detected in cultured L929 fibroblasts, rat dermal fibroblasts, and sciatic nerve Schwann cells, as well as in rat skin. Medium conditioned by cultured fibroblasts and Schwann cells also stimulates neurite growth from retinal explants and promotes the su...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90265-2
更新日期:1991-08-01 00:00:00
abstract::Several cDNAs encoding an isoform of the alpha 1 subunit of the voltage-dependent calcium channel were isolated from rat brain cDNA libraries. The complete nucleotide sequence of 6975 bp encodes a protein of 1634 amino acids, which corresponds to an Mr of 186,968. The protein exhibits 71% and 76% homology to skeletal ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90072-8
更新日期:1991-07-01 00:00:00
abstract::Uptake and transneuronal passage of wild-type and attenuated strains of a swine alpha-herpesvirus (pseudorabies [PRV]) were examined in rat visual projections. Both strains of virus infected subpopulations of retinal ganglion cells and passed transneuronally to infect retino-recipient neurons in the forebrain. However...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90236-s
更新日期:1991-06-01 00:00:00
abstract::AMPA but not kainate produces a rapidly desensitizing response in mouse hippocampal neurons. The characteristic action of these agonists appears to arise from activation of a single receptor with active and desensitized states, for which AMPA and kainate have different relative affinity. The equilibrium potency of a s...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90175-y
更新日期:1991-05-01 00:00:00
abstract::Evolutionary conservation of members of the NGF family in vertebrates was studied by DNA sequence analysis of PCR fragments for NGF, BDNF, and NT-3 from human, rat, chicken, viper, Xenopus, salmon, and ray. The results showed that the three factors are highly conserved from fishes to mammals. Phylogenetic trees reflec...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90180-8
更新日期:1991-05-01 00:00:00
abstract::Recent binding studies in the central nervous system and other tissues provide evidence that the mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin-B (NMB), exert their numerous physiological effects through at least two different receptors. We describe the structure and expression of a c...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90250-4
更新日期:1991-03-01 00:00:00
abstract::To define the cis-acting DNA elements required for rhodopsin expression, we generated lines of transgenic mice carrying sequences upstream of the bovine rhodopsin gene fused to the E. coli beta-galactosidase gene (lacZ). Upstream sequences extending from -2174 to +70 bp, from -734 to +70 bp, and from -222 to +70 bp di...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90355-4
更新日期:1991-02-01 00:00:00
abstract::Phorbol esters and purified protein kinase C (PKC) have been shown to down-modulate the voltage-dependent Na+ channels expressed in Xenopus oocytes injected with chick brain RNA. We used the two-electrode voltage-clamp technique to demonstrate that a Na+ channel expressed in oocytes injected with RNA coding for the al...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90131-i
更新日期:1991-01-01 00:00:00
abstract::Neuron B19 of Helisoma is selective in synaptogenesis. Presynaptic mechanisms underlying this selectivity were tested. Acetylcholine-sensitive assay cells were micromanipulated into contact with B19 somata to assess its secretory state. Prior to appropriate muscle target contact, spontaneous synaptic currents were det...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90129-n
更新日期:1991-01-01 00:00:00
abstract::We have examined the influence of retinal innervation on the development of target neurons in the first optic ganglion, the lamina, of D. melanogaster. Mitotically active lamina precursor cells (LPCs), which normally produce lamina neurons, are absent in mutants that lack retinal innervation, while other proliferative...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(91)90124-i
更新日期:1991-01-01 00:00:00
abstract::The sympathetic innervation of rat sweat glands undergoes a target-induced switch from a noradrenergic to a cholinergic and peptidergic phenotype during development. Treatment of cultured sympathetic neurons with sweat gland extracts mimics many of the changes seen in vivo. Extracts induce choline acetyltransferase ac...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90350-o
更新日期:1990-12-01 00:00:00
abstract::GABA, the major inhibitory neurotransmitter in the mammalian brain, binds to GABAA receptors, which form chloride ion channels. The predicted structure of the GABAA receptor places a consensus phosphorylation site for cAMP-dependent protein kinase (PKA) on an intracellular domain of the channel. Phosphorylation by var...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90338-g
更新日期:1990-12-01 00:00:00
abstract::Na+ channels are present at high density in squid giant axon but are absent from its somata in the giant fiber lobe (GFL) of the stellate ganglion. GFL cells dispersed in vitro maintain growing axons and develop a Na+ channel distribution similar to that in vivo. Tunicamycin, a glycosylation inhibitor, selectively dis...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90220-a
更新日期:1990-11-01 00:00:00
abstract::Cell proliferation during successive stages of oligodendrocyte development was delineated in the rat brain and optic nerve. Surface antigens, A2B5, O4, and galactocerebroside (GalC) identified three cell populations emerging in sequence; the incorporation of bromodeoxyuridine into newly synthesized DNA identified the ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90216-3
更新日期:1990-11-01 00:00:00
abstract::Modulation of evoked quantal transmitter release by protein kinase C (PKC) was investigated at an identified cholinergic neuro-neuronal synapse of the Aplysia buccal ganglion. Evoked acetylcholine release was increased by a diacylglycerol analog that activates PKC and was decreased by H-7, a blocker of PKC. FLRFamide ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90087-v
更新日期:1990-10-01 00:00:00
abstract::Fibroblastic 3T3 and endocrine RIN cells were genetically modified by infection with a recombinant retrovirus encoding the form I of human tyrosine hydroxylase (TH) and selection in tyrosine-free medium. These cells were grafted to rats unilaterally lesioned with 6-hydroxy-dopamine. Both cell types survived implantati...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90078-t
更新日期:1990-10-01 00:00:00
abstract::Spinal cord axons display a rostrocaudal, positional bias in their innervation of sympathetic ganglia and intercostal skeletal muscles. In an effort to examine the molecular basis of this positional specificity, we used the cyclophosphamide immunosuppression method to produce monoclonal antibodies that bind preferenti...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90081-p
更新日期:1990-10-01 00:00:00
abstract::We have studied the subcellular distribution of the alpha 1 and alpha 2 subunits of the dihydropyridine (DHP) receptor and ankyrin in rat skeletal muscle with immunofluorescence and immunogold labeling techniques. All three proteins were concentrated in the triad junction formed between the T-tubules and sarcoplasmic ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90170-k
更新日期:1990-09-01 00:00:00
abstract::In the developing mammalian visual system, retinal fibers grow through the optic chiasm, where one population crosses to the opposite side of the brain and the other does not. Evidence from labeling growing retinal axons with the carbocyanine dye Dil in mouse embryos indicates that the two subpopulations diverge at a ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90307-2
更新日期:1990-08-01 00:00:00
abstract::We constructed a recombinant baculovirus, A. californica nuclear polyhedrosis virus, containing the Drosophila Shaker H4 K+ channel cDNA under control of the polyhedrin promoter. When infected with this recombinant baculovirus, the cell line Sf9, derived from the army-worm caterpillar S. frugiperda, expresses fully fu...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90311-3
更新日期:1990-08-01 00:00:00
abstract::In situ hybridization histochemistry reveals localized expression of the nicotinic acetylcholine receptor (nAChR) alpha 2 subunit mRNA restricted to the lateral spiriform nucleus (SpL) of the chick diencephalon. The alpha 2 nAChR transcripts are not detected in immature SpL neurons at 4.5-5 days of embryonic developme...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(90)90032-b
更新日期:1990-07-01 00:00:00